Uganda can qualify for the World Cup in Russian Federation if they beat Ghana and Congo Brazzaville and hope that Egypt falters in one of their games.
He was expected to return for the clash against the Black Stars but due to a tight work schedule, he says he won't make it.
Appiah added, "We have players in this squad, who deserve to play".
Ghana's Black Stars left Kenya on Thursday for Uganda ahead of the crucial 2018 World Cup qualifier in the Ugandan capital Kampala at the weekend. It will all be over should Ghana and Egypt win their penultimate games as that brings Ghana to eight points and Egypt, whom the play in the last round of matches, to 12 points.
Ghana will now face the cranes of Uganda without the services the three key players.
This comes at a time when coach James Appiah Kwesi dropped brothers Andre Ayew and Jordan Ayew, claiming he wanted to give chance to upcoming players.
Uganda are now second on the Group E log on seven points, two shy of group leaders Egypt who host bottom side Congo on Sunday.
"We want to go into the game prepared well because it is very vital for both of us at this stage".
Basena replaced Serbian tactician Milutin Sredojevi? early this year and has masterminded Uganda's impressive run in the 2018 World Cup qualifiers where and have a realistic chance of making it to Russian Federation at the expense of Egypt and Ghana.
"We were hoping to get the full house but unfortunately Thomas Agyepong, Harrison, Jonathan Mensah, Asamoah Gyan and Afriyie Aquah are all out".